Midterm
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Conductance
Siemens ( S )
Activity( radionuclide )
Becquerel ( Bq )
Resistance
Ohms
Substance conc.
Mols per cubic meter
Volume
Cubic meter
Are obtain by combining 2 or more units by multiplication or division
SI derived units
States that the analyze is directly proportional to the amount of light it absorbs
Beer’s law
Is the term used to describe the monochromatic light this is absorbed by the sample and transmittance describes the light that passes through the sample
Absorbable ( OD )
Visual colorimetry which solely on the human eye
Dubosq colorimeter
A device for selecting a narrow band of wavelengths from a continuous spectrum
Monochromator
When the absorbance increases exponentially with an increase in the light path this law is followed
Lambert law
Incorporation of these two laws together with the contribution of blank
Bouguer
Formula of absorbance OD
A= 2 - log%T
Continuous spectrum examples
Filter ,Prism and Diffraction grating
Photoelectric colorimetry which employs a photoelectric device
Leitz photrometer
LambertlAw and contribution of bouguer
A= abc A= is for absorbance a= absorptivity of the substance being measured b= light in cm c= conc. of the measured substance
Beer-Lambert law formula
Au x Cs /As = Cu
Two types of beer law
Duboscq colorimeter and Lietz photrometer
This formula applied to assay exhibit linear relationship between changes in absorbance with changes in conc to calculate the conc of unknown
Au x Cs /As = Cu
It minimizes stray light emitted by lamp and prevent scattered light from entering the monochromator
Entrance slit
Does not provide a continuous spectrum ,emitting radiation at a specific wavelength
Mercury vapor lamp
It controls the amount of emergent light that passes through the event
Exit slit
This provide the radiant energy
Light source
NIR
Near infrared