lab exam 1
No description yet. This curated deck is ready for daily study.
Card preview (24)
what goes in biohazard container
live container, liquid/solid culture,gloves
what goes in the general trash
paper towels, Kimwipes, paper and gloves
what goes in plastic pipettes
pl;astic loops, plastic needles
What is the objective of Aspetic Teechnique?
transfer of bacteria from one medium to another without introducing contaminants
What is the objective of subculturing
the transfer of microorganism from one medium to another for fresh growth
what is a pure culture
population of a single species
what is a mixed culture
population containing two or more species
streak plate is also called
streaking for singles
hwo do you streak plate
essentially a dilution, created by a loopful of culture over the surface of an agar plate
how do you spread plate
microorganism spread evenly throughout the plate
describe pseudomonas aeruginosa
ROD, can be pigmented, major cause of lung infection with cystic fibrous
describe staph, aureus
COCCUS, harmless skin bacteria to MRSA(pathogen)
Describe bacillus cereus
ROD, spore former, soil bacteria can cause food poisoning
which bacteria can be milky white to golden
S.aureus
purpose of bacterial smear
can be stained so as to better view the bacteria
what does a stain consist of
benzene ring, a chromophore, auxochrome
what does a chromophore do
imparts color
what does auxochrome
ionizes the chromogen
is a basic stain positively or negatively charged
positively charged
is a acid stain positively or negatively charged
negatively charges
examples of basic stains
methylene blue, carbon fuschin and crystal violet
correct exposure for methylene blue
1-2 min
correct exposure for crystal violet
20-60 secs
correct exposure for carbol fuchsin
15-30 sec