EXPERIEMT #14 FINALS
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What are we talking about in experiment 14?
Albumin Total serum proteins A/G ratio
How are you going to correlate test results with pathologic conditions?
Electrophoresis pattern
What are you going to explain in Experiment #14?
Amino acid synthesis and metabolism
What are you going to discuss in Experiment #14?
The different types of aminoacidopathies
In Serum Albumin Determination, what method is used?
Bromocresol Green Method (BCG)
What is the principle of Serum Albumin Determination?
The binding behavior of the protein with an anionic dye.
Principle of Serum Albumin Determination: Determination of __________ in ____________is usually based on the ___________________, _____________ forming a ____________.
Albumin serum or plasma binding behavior of the protein with an anionic dye bromcresol green green colored complex.
What is the concentration standard of Total protein?
6g/dL
What is the concentration standard of Serum Albumin?
4g/dL
What is the formula for computing Total protein and serum standard?
Cu= Au/Astd x Cstd
How much serum albumin reagent is added?
3.0mL
To which tube is serum albumin reagent added?
Blank Standard Sample
How much protein standard is added?
3.0mL
To which tube protein reagent is added?
Blank Standard Sample
How much and to which tube water is added for both serum and Albumin determination?
20uL Blank
How much and to which tube STANDARD is added for both serum albumin and total protein determination?
20uL Standard sample
How much and to which tube SERUM is added for both serum Albumin and total protein determination?
20uL Sample
In performing Albumin, total protein determination, what specimen is used?
Serum
In SAD and Total Protein, at what temperature are we going to stand the samples?
Room temperature
Under SAD, at what absorbance are we going to read the blank, standard and sample?
600nm
Under SAD, how much time is its stability?
60 MINUTES / 1 HR
In determination of Total Proteins, what method is used?
Biuret (Modified)
What is the principle of Biuret Method?
The copper binds to the peptide bond structure of the protein.
When a solution containing proteins is rejected with a tartrate-complex cupric ions and alkali, the copper binds to the peptide bond structure of the proteins. What is the color that is produced?
Purple colored chromogen