exam 3
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which organelles are distinct compartments separated from the cytosol by at least 1 selectively permeable membrane?
• nucleus • endoplasmic reticulum • golgi apparatus • lysosomes • endosomes • mitochondria • peroxisomes
cytosol functions
• contains many metabolic pathways • protein synthesis • the cytoskeleton
nucleus functions
• contains many genome • DNA and RNA synthesis
endoplasmic reticulum functions
• synthesis of most lipids • synthesis of proteins for distribution to many organelles and to the plasma membrane
golgi apparatus functions
• modification, sorting, and packaging of proteins and lipids for either secretion or delivery to another organelle
lysosome functions
• intracellular degradation
endosome functions
• sorting of endocytosed material
mitochondria functions
• ATP synthesis by oxidative phosphorylation
chloroplast functions
• ATP synthesis and carbon fixation by photosynthesis
peroxisome functions
• oxidative breakdown of toxic molecules
how do proteins get in to the different compartments?
protein sorting
proteins are transported into organelles by what 3 mechanisms?
• nuclear import • across a membrane • vesicular
what directs proteins to the correct compartments?
signal sequences
what happens of a protein does not have a signal sequence?
it will remain in the cytosol
(vocab) chains of amino acids that bind to proteins and direct them
signal sequences
(vocab) recognize nuclear localization signal (NLS)
nuclear import receptors
nuclear proteins are imported from the cytosol through _______________________
nuclear pores
why does nuclear import receptor release cargo protein once it enters the nucleus?
Ran (GTP binding protein) binds to import receptor in nucleus, causing cargo protein to be released
Ran can only bind to import receptors when bound to what?
GTP
location of Ran GDP
cytoplasm
location of Ran GDP
nucleus
what is the full name for Ran-GEF
Ran Guanine Exchange Factor
what is the TOM
translocator of outer membrane
what is the TIM
translocator of inner membrane