Microscopy
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What does a microscope enable us to do?
It enables the human eye to visualise objects which are magnified using optical lenses
List the ten key components of a light microscope.
Light source Condenser Substage diaphragm Specimen stage Objective lens Body tube Eyepiece lens Focus adjuster dials Filters - optional Power switch
What is the eyepiece also called?
Ocular lens
What is darkfield microscopy? 3
A darkfield-condenser blocks out most light Light is scattered by particulate objects, these are seen as luminous objects Specimen appears as white on a black background
What is phase contact microscopy? 2
Phase contrast microscope uses two annular rings to slow light, before it is recombined, This enhances the image of denser components
What is differential Interference Contrast Microscopy? (2)
Differential interference microscopy uses polarizing prisms to enhance density differences and give a 3D image. Its similar to phase contrast.
What is fluorescence microscopy? 3
In fluorescence microscopy light is absorbed by molecules, Electrons are moved to higher energy level. Electrons returning to lower energy level emit energy as Light – visualised as fluorescence
What is electron microscopy? 3
Electron beam instead of light source- Electrons hit photomultiplier tube, This emits an electrical signal, which is captured creating an image