Chromotagraphy exam qs

State how the amino acids can be made visible at the end of the experiment.

State why each amino acid has a different Rf value

Suggest a suitable reagent for the hydrolysis of a protein.

Suggest how the positions of the amino acids on the TLC plate were located

Suggest why it was necessary to use two different solvents

Outline the steps needed to locate the positions of the amino acids on the TLC plate and to determine their Rf values.

Explain why different amino acids have different Rf values. 2 marks

To obtain the chromatogram, the TLC plate was held by the edges and placed in the solvent in the beaker in the fume cupboard. The lid was then replaced on the beaker. Give one other practical requirement when placing the plate in the beaker

A third TLC experiment was carried out using 1,2‑dinitrobenzene. An identical plate to that in part (c) was used under the same conditions, but the solvent used contained a mixture of hexane and ethyl ethanoate. A student stated that the Rf value of 1,2‑dinitrobenzene in this third experiment would be greater than that of 1,2‑dinitrobenzene in the experiment in part (f) Is the student correct? Justify your answer.

Describe a test-tube reaction, on the product, to show that the cyclohexanol had been dehydrated. State what you would observ

Explain why it is important to open the tap of the separating funnel periodically.

Give a property of anhydrous calcium chloride, other than its ability to absorb water, that makes it suitable as a drying agent in this preparatio

Explain why cyclohexene has a shorter retention time than cyclohexanol.