FISH article

multicolor FISH assay for detection of chromosomal abnormalities in sperm cells

42-color M-FISH variant that allows the detection of chromosomal abnormalities in the p- and q-arms of all 24 human chromosomes, except the p-arm of the Y and acrocentric chromosomes

very useful for detection, identification, and quantification of microorganisms involved in bioleaching processes

used to determine the interactions of neuronal populations associated with different behaviors

involves hybridization on binucleated cells in which cytokinesis has been blocked by treatment with cytochalasin B (CB)

allows to determine the relative orientation of two or more DNA sequences along a chromosome; useful in assessing chromosomal translocations and inversions

enables recognition of all human chromosome arms on the basis of color and mapping of gene and viral integration site in the context of chromosome arm painting

This protocol is similar to CO-FISH except for the information about the directional organization of telomeric sequences.

used for specific labeling of genomic sites. It takes advantage of homopurine/homopyrimidine oligonucleotides that form triple helices with intact duplex genomic DNA.

combination of comet assay and FISH analysis. It is used to detect genome region-specific DNA damage. successfully used to determine the sensitivity of telomeres to damage.

makes use of ultrathin cryosections (150 nm thick) of sucrose-embedded cells. The spatial interrelationship of chromosome territories and the genome organization in the cell nucleus has been successfully studied with this technique.

In this FISH, a secondary color is observed since the adjacent colors overlap.

used to determine DNA fragmentation levels in sperms

BLAST-based FISH simulation program, which can predict the out- come of hybridization experiments

a technique in which DNA fibers or chromatin fibers are released from cell nuclei by salt or solvent extraction and stretched on a microscope slide prior to hybridization

in situ hybridization is combined with flow cytometry for measurement of the telomeric signals from cells in suspension

initially used for identification of the 9;22 Philadelphia translocation in peripheral blood and bone marrow cells of CML patients to detect minimal residual disease after bone marrow transplantation

the cells are first permeabilized and then extracted with high salt to remove soluble protein

a method for cell cycle-controlled chromosome analysis in human lymphocytes that allows a precise quantification of induced chromosome damage for human biodosimetry

a combination of standard FISH and indirect or direct immuno- fluorescence. With this technique, the antigens can be visualized within the sample.

more useful for the detection of mRNA and genes on the chromosomes

the ability to identify several regions or genes simultaneously using different colors; used to analyze specific chromosomal subregions, like centromeres and sub-centromeres

refers to the simultaneous use of multiple probes in multicolor FISH. This FISH assay was initially designed to screen for multiple microdeletion syndromes in patients with unexplained developmental delay and/or mental retardation.

first used to measure individual telomere lengths on metaphase chromosomes