AMS
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What is the systematic name of amylase?
E.C.3.2.1.1
What is the chemical name of AMS?
a-1,4-glucan-4-glucanohydrolase
What does the amylase hydrolyze?
1,4-a-glucocidic linkage in polysaccharides.
The only enzyme that can be found in the urine
Amylase
What is the MW of amylase?
45,000
What is the optimum pH of Amylase?
Optimum pH: 6.9 – 7.0 in serum
Without this, amylase cannot perform its duty.
Calcium
Where is the amylase secreted in the pancreas?
Acinar cells
Responsible in the hydrolysis of starch from the food that we eat.
Salivary gland
Small amount of Amylase can be found
SEMINAL FLUID, TESTIS, OVARIES, FALLOPIAN TUBES, STRIATED MUSCLE, LUNGS AND ADIPOSE TISSUE
first milk produced by a lactating mother
Colostrum
it hydrolyses the 1,4-a-glucocidic linkage to form the following 3 products:
a. Glucose b. Maltose c. Dextrin
What are the reactions catalyzed by amylase?
Starch digestion Splits complex CHO made up of a-D glucose units
Inhibited by monoclonal antibody Inhibited by protein isolated from wheat Accounts for the 2/3 of the Amylase activity in the normal serum
S-type Isoamylase
Fastest migrating in electrophoresis
S1 isoamylase
Inhibited by monoclonal antibody Not inhibited by protein isolated from wheat
P-type Isoamylase (Pancreas)
What is the most common fraction type of isoenzyme?
P2, S1 AND S2
What p-type isoenzyme is predominant in cases of acute pancreatitis?
P3
Abnormal amylase in combination with Immunoglobulins (IgA or IgG) or other high MW proteins can also bind to Stype isoenzyme
Macroamylase
What specific immunoglobulins do abnormal amylase combine to?
IgA OR IgG
Sudden inflammation of the pancreas causing abdominal pain.
Acute pancreatitis
Predisposal for acute pancreatitis
Alcoholism
Cause of ACUTE PANCREATITIS?
Gallstone formation
Viral infection (Paramyxoviridae) that mainly effect the parotid gland and the salivary gland
Mumps