chromatography
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TLC chromaptography?
a plate is coated with a solid and a solvent moves up the plate
CC chromatography ?
a column is packed with a solid and a solvent moves down the column
GC chromatography ?
a column is packed with a solid or with a solid coated by a liquid, and a gas is passed through the column under pressure at high temperature
what does seperation in column chromatography depend on?
balance between solubility in the moving phase and retention in the stationary phase
solid stationary phase separates by ….
adsorption
liquid stationary phase separates ….
relative solubilty
what is Chromatography
an analytical technique that separates components in a mixture between a mobile phase and a stationary phase
what is the mobile phase in GC
an inert gas
what is the stationary phase in GC
a liquid on an inert solid
what do you use if the spots are colourless and not visible
UV lamp
what is HPLC’s stationary phase
solid silica
what is HPLC’s mobile phase
a liquid
why is Gas-Column chromatography used
to separate mixtures of volatile liquids
what is GC-MS used for
forensics, environmental analysis, airport security and space probes