LIPIDS AND LIPOPROTEINS part 3
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What are the interferences of Enzymatic method?
Elevated levels of ascorbic acid; hemoglobin and bilirubin
What value causes the bilirubin to decrease cholesterol?
5 mg/dL
If Bilirubin exceeds 5mg/dL it decreases cholesterol by how many percent?
5-15%
What enzyme acted on cholesterol ester and water to form cholesterol and fatty acid?
Cholesterol esterase
What enzyme acted on Cholesterol and oxygen to produce Cholest-4-en-one and hydrogen peroxide?
Cholesterol Oxidase
What enzyme acted on hydrogen peroxide and 4-aminanotipyrine to produce quinoneimine dye?
Peroxidase
4-anminoantipyrine is replaceable by ___________ on the third step.
Methanol
In the presence of peroxidase, what happens to methanol?
It is oxidized into formaldehyde
It is used in detecting acetylaceton
Formaldehyde
In other methods, Cholest-4-en-3-one or cholesterol is measured at?
240nm
The absorbance of Quinoneimine dye is measured at?
500nm
4-aminoantipyrine is AKA as?
4-aminophenazone
this uses cholesterol ester, hydrolase, and cholesterol oxidase
Oxygen consumption
What causes the formation of water and oxygen under oxygen consumption?
Hydrogen peroxide is mixed with peroxidase
this measures the oxygen released after the decomposition of hydrogen peroxide
Oxygen electrode
It is not easily automated unlike others, it generally requires a lot of cholesterol oxidase
Oxygen consumption under cholesterol
Oxygen consumption under cholesterol requires a lot of?
Cholesterol oxidase
How many molecules of FA and Glycerol do TGC contain?
3 1
The molecules of FA and Glycerol is held by what bond?
Ester bond
What is the characteristic of TGC?
Hydrophobic and water insoluble
main storage formof lipid
TGC
Where is TCG found?
Adipose tissues
Where is TCG from?
Plant sources rich in unsaturated FA
TCG from animal sources contains?
Saturated FA which are solid at room temp