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Introduction to Gene Cloning

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Total cards: 10•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (10)

#1
Front (Question)

Why is it a challenge for molecular biologists?

Back (Answer)

• The cell has 1000s of macromolecules and has a genome containing millions of base pairs • Powerful tools are needed for genetic analysis

#2
Front (Question)

How many genes do E.coli have?

Back (Answer)

> 2000

#3
Front (Question)

How many base pairs does E.coli have?

Back (Answer)

4,600,000

#4
Front (Question)

How many genes do humans have?

Back (Answer)

25,000

#5
Front (Question)

How many base pairs do humans have?

Back (Answer)

3000 million

#6
Front (Question)

A human gene may only constitute for only how much of a chromosonal DNA molecule?

Back (Answer)

1/100000

#7
Front (Question)

What is recombinant DNA (rDNA)?

Back (Answer)

DNA recombined from two different sources

#8
Front (Question)

What do cloning vectors and recombinant DNA allow scientists to do?

Back (Answer)

Modify specific genes and move them from one organism to another

#9
Front (Question)

What is the aim of cloning vectors and recombinant DNA?

Back (Answer)

Produce large quantities of the gene of interest or its protein product

#10
Front (Question)

Name the 4 objectives of recombinant DNA technology

Back (Answer)

• Identifying genes • Isolating genes • Modifying genes • Re-expressing genes in other hosts or organisms