lab quiz 1
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all laboratory safety information and policies
goggles, no eating or drinking, tie back hair, PPE, and clean work area before and after
laboratory safety behavior and personal protective equipment
goggles, gloves, lab coat, full length shirts and pants, closed toe shoes
proper clean-up and handling procedures
do not return chemicals to container, ensure labware is clean
how to clean labware
soft cloth, warm water and soap, rinse with distilled water
How to dispose chemicals and non chemical substances
chemicals: contact local waste managment non chem: wrap in paper towel or newspaper
How to pick the best place in your home
Ventilated, non porous surface, uncluttered, near running water, heat tolerant surface NO PLASTIC, WOOD, OR GLASS
types of injuries and how to treat
Chem burns: flush skin with water and add baking soda, use first aid otherwise
Safety Data Sheets, what they contain and how to find them
Includes Info: properties of chemicals, physical, health, and environment hazards; protective measures and safety
where to find Safety Data Sheets
manufactures website or google
How to pour agar plates
heat up agar in water covering 1mm above the agar in tube with lid slightly off. Heat till translucent and smooth surface. pour evenly, let cool w/ top slightly off
Addie Rerecich’s Case
Bacteria: MRSA, 2nd infection: caused by ECMO cause: picking scab on playground treatment: lung transplant outlook: don’t know how long the lungs will last, everything is hard, takes pills, worried about bacteria
David Ricci’s Case
Bacteria: NDM-1 enzyme produced by the bacteria Cause: Train ran over his leg, lead to amputation Treatment: daily surgery and colinstin outlook: disease will never go away, it is just dormant in his body
NIH in Maryland
Gene found: KPC found in digestive system of animals 18 patients, 6 dead couldn’t get rid of NIH precautions: wear gloves, gowns, separated patients,created separate ICU, used robots, aseptic techniques
Why do antibiotics become less resistant?
Overuse Misuse Not finishing prescriptions
Why are there not a lot of antibiotics being produced
Antibiotic resistance is more common and they don’t want to spend $$ on creating new ones
How many infections there are in the world and if that number is accurate
We don’t know, there is no uniform reporting mechanisms or data.
Aseptic techniques
hand wash, 10% bleach clean surfaces, sterilize equipment
What are the different cultures
Mixed Culture: contain many types of microbes Pure culture: contains species of microbe contaminated culture: unknown microbes are exposed
Isolation techniques
streak plates
Benefit of streak plates
dilutes microbes, easier to see morphology
Shape of E. Coli, Saccharomyces cerevisiae, staphylococcus epidermis
all circluar, entire margin, convex, and small-moderate size
Bacterial Morphology
coccus, bacillus, spirilla
How do Cocci arrange together
Diplococci: daughter cells fail fully separate tetrads: remain in groups of 4 sarcina: cubical packets, 8 cells-16 cells
How do bacili arrange together
diplobacilli: end to end streptobacilli: side by side