[LAB] LDH
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REFERENCE METHOD OF LDH
KINETIC METHOD BY BUHL AND JACKSON MODIFIED BY WACKER
MAJOR ORGANS WHERE LDH IS DISTRIBUTED IN
HEART LIVER MUSCLE KIDNEY
ELEVATED LEVELS OF SERUM LDH IS FOUND IN WHAT CLINICAL CONIDITONS
MI LIVER DISEASE RENAL DISEASE ANEMIA MALIGNANT DISEASES PROGRESSIVE MUSCLE DYSTROPHY
IN WHAT DIRECTION CAN THE LD ENZYME BE MEASURED
FORWARD REVERSE
[ADVANTAGES] LACTATE TO PYRUVATE METHOD
BETTER REAGENT STABILITY ELIMINATION OF PRE-INCUBATION LINEAR RATE OF REACTION LESS CONTAMINATION OF NAD
PRINCIPLE OF LDH
L-LACTATE + NAD <—LDH (PH 8.3 TO 9.0) —> PYRUVATE + NADH + H
COENZYME OF LDH
NAD
DOES LDH ACT ON D-LACTATE
NO
WHAT HAPPENS TO PYRUVATE AFTER FORMATION
PROCEED TO THE EMBDEN-MEYERHOFF PATHWAY FOR THE GENERATION OF ENERGY
SOLUTIONS MIXED WITH NADH IN THE WACKER COLORIMETRIC METHOD
PHENAZINE METHOSULFATE NITROBLUE TETRAZOLIUM
END PRODUCT OF THE WACKER COLORIMETRIC METHOD
BLUE COLORED COMPOUND
RELATIONSHIP OF THE ABS TO AMOUNT OF NADH AND LD ACTIVITY IN THE WACKER COLORIMETRIC METHOD
DIECTLY PROPORTIONAL
[DIRECTION OF REACTION] COLORIMETRIC METHOD BASED ON WACKER
LACTATE TO PYRUVATE
[DIRECTION OF REACTION] WROBLEWSKI AND LA DUE
PYRUVATE TO LACTATE
PH OF THE REACTION IN THE WROBLEWSKI AND LA DUE METHOD
PH 7.4 TO 7.8
RELATIONSHIP OF NADH BOLUME ANDNLD ACTIVITY IN THE WROBLEWSKI AND LA DUE METHOD
INDIRECTLY PROPORTIONAL
DIFFERENCE IN THE SPEED OF REACTION IN THE FORWARD AND REVERSE REACTION IN THE WROBLEWSKI AND LA DUE METHOD
REVERSE IS THREE TIMES FASTER
[ADVANTAGES] WROBLEWSKI AND LA DUE METHOD
SMALLER SAMPLE VOLUMES SHORTER REACTION TIME
[DISADVANTAGES] WROBLEWSKI AND LA DUE METHOD
SUSCEPTIBLE TO SUBSTRATE EXHAUSTION SUSCEPTIBLE TO LOSS OF LINEARITY
METHODS FOR SEPARATION AND QUANTITATION OF LDH ISOENZYMES
ELECTROPHORESIS COLUMN CHROMATOGRAPHY IMMUNO-INHIBITION BABSON’S METHOD KAPLAN AND BERER USE OF ALPHA HYDROXYBUTYRATE AS SUBSTRATE
ELECTROPHORETIC MIGRATION OF THE LD ISOENZYMES
FASTEST — LDH1 SLOWEST — LDH6 BETWEEN LDH3/4 — LDH BOUND TO IMMUNOGLOBULIN (IgA/G)
LDH ISOENZYME ISOLATED IN THE COLUMN CHROMATOGRAPHY METHOD
LDH1 LDH2
METHOD USED FOR THE SCREENING OF AMI
COLUMN CHROMATOGRAPHY
LDH ISOENZYME ISOLATED IN THE IMMUNO-INHIBITION METHOD
LDH1