Direct Bilirubin

Diagnostic reagent for quantitative in vitro determination of direct bilirubin in human serum or heparin plasma on automated photometric systems

Photometric test using 2,4-dichloroaniline (DCA) Direct bilirubin in presence of diazotized 2,4-dichloroaniline forms a red colored azocompound in acidic solution.

EDTA-Na2 - 0.1 mmol/L NaCl - 150 mmol/L Sulfamic acid - 100 mmol/L

2,4-Dichloroaniline - 0.5 mmol/L HCl - 900 mmol/L EDTA-Na2 - 0.13 mmol/L

H290 - may be corrosive to metals P234 - keep only in original packaging P390 - absorb spillage to prevent material damage

• In very rare cases, samples of patients with gammopathy might give falsified results • Eltrombopag medication leads to falsely low or high results in patient samples. • Please refer to the safety data sheets and take the necessary precautions for the use of laboratory reagents. For diagnostic purposes, the results should always be assessed with the patient’s medical history, clinical examinations and other findings

Human serum or heparin plasma

2 days at 20 – 25°C 7 days at 4 – 8°C 6 months at –20°C in case of immediate freezing.

545/658 nm

37°C

Endpoint

3.5 µL

80 µL

20 µL

Cycle 19 (286 s)

Cycle 17/18 (230/244 s)

Cycle 41/42 (586/600 s)

Linear

DiaSys TruCal U

Jendrassik-Gróf test

DiaSys Trulab N and P

Diluted Calculation: 1 + 1 with NaCl solution (9 g/L) and the result multiplied by 2

0.01 mg/dL

Ascorbic acid - 30 mg/dL Hemoglobin (interferes at low concentration) Lipemia (TAG) - 600 mg/dL