MIDTERM LEC 1.3
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CALIBRATION MARKS/DESIGN It delivers the exact amount it holds into a container; should not be blown out
To Deliver (TD) Pipette
CALIBRATION MARKS/DESIGN It is designed to meet the requirements of Class A-type pipettes.
To Deliver (TD) Pipette
CALIBRATION MARKS/DESIGN It is designed to drain by gravity, and the desired volume is obtained when draining stops.
To Deliver (TD) Pipette
CALIBRATION MARKS/DESIGN Proper usage: Hold vertically with the tip against the side of the container and should not touch the accumulating liquid
To Deliver (TD) Pipette
CALIBRATION MARKS/DESIGN To Deliver (TD) Pipette Examples:
Serologic, Mohr, and volumetric transfer pipettes
CALIBRATION MARKS/DESIGN It holds a particular volume but does not dispense the exact volume.
To Contain (TC) Pipette
CALIBRATION MARKS/DESIGN It is also known as the rinse-out pipette.
To Contain (TC) Pipette
CALIBRATION MARKS/DESIGN It does not meet Class A certification criteria.
To Contain (TC) Pipette
CALIBRATION MARKS/DESIGN To Contain (TC) Pipette
Sahli hemoglobin and Lang-Levy pipettes
DRAINAGE CHARACTERISTICS It has continuous etched rings on top or near the mouthpiece of the pipette and exact volume is obtained when the last drop is blown out.
Blow-out Pipette
DRAINAGE CHARACTERISTICS This type of pipette is not rinsed out.
Blow-out Pipette
DRAINAGE CHARACTERISTICS Blow-out Pipette Examples:
Ostwald-Folin and serological pipette
DRAINAGE CHARACTERISTICS ● It allows the liquid to drain by gravity
Self-draining
DRAINAGE CHARACTERISTICS It does not have etched or frosted rings
Self draining
Transfer Pipette for nonviscous fluid; self-draining; small amount left in the tip should not be blown out
Volumetric Pipette
Transfer Pipette for viscous fluid; with etched ring
Ostwald Folin
Transfer Pipette transfers fluids without consideration of a specific volume
Pasteur Pipette
Graduated or Measuring Pipette with graduations to the tip; blow-out pipette
Serological Pipette
Graduated or Measuring Pipette without graduations to the tip; calibrated between 2 marks; self-draining Pipette
Mohr Pipette
Graduated or Measuring Pipette TC pipettes - Glass Micropipettes (< 1 mL) (4)
Sahli-Hellige Pipette Lang-Levy Pipette RBC and WBC Pipettes Kirk and Overflow Pipettes
It relies on piston for suction creation to draw the sample into a disposable tip.
Air Displacement Pipette
The piston does not come in contact with the liquid
Air Displacement Pipette
A disposable, one-time use polypropylene tip is attached to the pipette barrel.
Air Displacement Pipette
It operates by moving the piston in the pipette tip or barrel, much like a hypodermic syringe.
Positive Displacement Pipette