Exam 3
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What are the major electron carriers in the ETC?
flavoproteins, iron-sulfur proteins, cytochromes, copper-containing cytochromes, coenzyme Q
GLY-1 reaction + explanation
Glucose → G-6-P - Rxn is highly exergonic and powered by ATP - Glucose gets phosphorylated to make G6P
GLY-2 reaction + explanation
G-6-P ⇌ F-6-P - G6P gets rearranged into fructose
GLY-3 reaction + explanation
F-6-P ⇌ F-1,6P -Rxn is highly exergonic and powered by ATP, leaving ADP and H⁺ leftover - Fructose makes fructose bisphosphate
GLY-4 reaction + explanation
F-1,6P ⇌ Dihydroxyacetone + G3P - F-1,6P is split into two isomers - 1st G3P molecule is made
GLY-5 reaction + explanation
Dihydroxyacetone ⇌ G3P - Dihydroxyacetone from GLY-4 makes the 2nd G3P molecule
GLY-6 reaction + explanation
2 G3P ⇌ 2 1,3-bisphosphoglycerate - NAD⁺ is reduced to NADH, NAD⁺ acts as an electron acceptor
GLY-7 reaction + explanation
2 1,3-BPG ⇌ 2 3-Phosphoglycerate - Makes 2 ATP via substrate level phosphorylation from ADP + Pi - Phosphate group comes from BPG
GLY-8 reaction + explanation
2 3-Phosphoglycerate ⇌ 2 2-Phosphoglycerate - 3 phosphoglycerate gets rearranged
GLY-9 reaction + explanation
2 2-Phosphoglycerate ⇌ 2 PEP - PEP gets hydrolyzed (water is a byproduct) - The most exergonic reaction in a biological system
GLY-10 reaction + explanation
2 PEP → 2 Pyruvate - Phosphate groups from PEP are joined with ADP to make ATP - 2 ATP molecules are synthesized via substrate level phosphorylation of ADP + H⁺
What is the overall reaction of glycolysis?
glucose + 2 NAD⁺ + 2 ADP + 2 Pi → 2 pyruvate + 2 NADH + 2 H⁺ + 2ATP
What is the net gain of ATP in glycolysis?
2 ATP
What is substrate level phosphorylation?
Direct transfer of a phosphate group from an organic substrate to ADP by an enzyme. Substrate level phosphorylation generates much less ATP during cellular respiration than oxidative phosphorylation.
When does substrate level phosphorylation occur?
During glycolysis and the citric acid cycle
How many ATP molecules does substrate level phosphorylation generate?
4 ATP molecules (2 ATP from glycolysis, 2 ATP per cycle of CAC)
What are the two types of anaerobic respiration?
lactic acid fermentation and alcoholic fermentation
What is the reaction of lactic acid fermentation?
2 pyruvate + 2 NADH ⇌ 2 lactate + 2 NAD⁺
What is lactic acid fermentation used for?
• Makes cheese and yogurt • Lactate is made when muscle cells undergoes lots of exercise, causing fatigue and soreness
What is the reaction for alcoholic fermentation?
2 pyruvate + 2 NADH + 4H⁺ → 2 ethanol + 2 CO₂ + 2 NAD⁺
Where does alcoholic fermentation occur?
Plant cells
What steps does phase 3 of glycolysis include?
Steps 8-10, Pyruvate formation and ATP generation
What steps of phase 1 of glycolysis include?
Steps 1-5, Preparation and cleavage
What steps does phase 2 of glycolysis include?
Steps 6-7, Oxidation and ATP generation