Chromatography

wear gloves and draw a pencil line above the bottom of the TLC plate mark spots for each sample on the line use a capillary tube to add tiny drops of each solution to a different spot allow the plate to air dry, then add solvent t o a large beaker wit a lid place TLC plate in beaker, ensuring solvent is below the pencil line place lid tihgtly to prevent toxic solvent rom evaporating remvoe plate when the level of solvent is near the top mark the solvent line with a pencil and allow it to dry in a fume cupboard place plate under UV light and use a pencil to draw lightly around the spots

no contamination of sweat which contains amino acids

makes colourless amino acid spots more visible

won’t dissolve in the solvent

Plate coated in silica gel

Mixture of amino acids

Silica gel packed inside a column Liquid solvent that flows through the column

High boiling point liquid adsorbed onto a solid Carrier gas (usually an inert gas like nitrogen)

allows us to identify and separate compounds in a mixture

how likely a substance i to be in the stationary or mobile phase

polar solvents

more corrosive/reactive substances would otherwise damage chromatography paper