Aplication Of DNA Tech
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What is DNA fingerprinting?
-DNA fingerprinting is a laboratory technique used to determine the probable identity of a person based on the nucleotide sequences of certain regions of human DNA that are unique to individuals
How is the nucleotide sequence used in DNA fingerprinting arranged?
• it is arranged in repeating sequences of base pairs (STRs - Short Tandem Repeats)
What type of DNA is used in DNA fingerprinting?
• the non-coding DNA is used as it differs from person to person
What are the uses in DNA profiling? (5)
• to identify crime suspects in forensic investigations • as proof of paternity • to determine the probability and cause of genetic defects • to trace missing persons • to establish the compatibility of tissue types for organ transplants
What is PCR?
• Polymerase Chain Reaction
What does PCR do?
• PCR replicates DNA because evidence samples often do not contain enough DNA for testing. • It can make millions of DNA segments from just a few copies of sample DNA
How does PCR replicate DNA segements?
• Through cycles of heating and cooling and using enzymes and nucleotides, the DNA fragments are replicated
What is the importance of PCR?
• is important in DNA fingerprinting as it ensures that there is enough DNA for testing
What is Gel Electrophoresis?
Gel Electrophoresis is a method that separates Nucleic acids according to their size