Bio chp. 9,12
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DNA stores…..
genetic material
What does DNA make and where is it stored and packaged?
makes polypeptides and RNA molecules. it is packaged in chromosomes and stored in nucleus
Name the 5 levels of organization of DNA structure
• Nucleotides • Single DNA strand • DNA Double helix • Chromosome • Genome
Nucleotides contain what 3 things?
phosphate, sugar, and a base
what are the 2 sugars?
Ribose in RNA Deoxyribose in DNA
What are the bases?
• Guanine (G) in DNA and RNA • Cytosine (C) in DNA and RNA • Adenine (A) in DNA and RNA • Uracil (U) only in RNA • Thymine (T) only in DNA
What is the nucleotide numbering system?
1’ to 5’ ▪ Phosphate group is attached to the 5’ carbon on the sugar ▪ Hydroxyl group (-OH) is attached to the 3’ carbon on the sugar
On a single strand, what links nucleotides and what does it form?
phosphodiester bonds link; forms a sugar phosphate backbone
Does a single strand have directionality
yes
Double helix definition
2 DNA stands associate together
What does the sugar phosphate groups form what?
backbone
Bases project towards the ______
center
What bonds are formed when bases project towards the center?
hydrogen bonds
The two strands are __________
antiparallel
DNA is wound around proteins to produce
chromatin
Chromatin
DNA + packaging proteins
What does it mean when DNA replication is semi conservative?
• Each new helix is composed of 1 original (parent) strand and 1 new (daughter) strand.
Where does DNA replication start?
origin of replication
What are the 4 DNA replication proteins?
• single stranded binding proteins • DNA topoisomerase • DNA helicase • DNA polymerase
Single strand binding proteins
Bind single stranded DNA to prevent it re-forming a double helix and to protect it
DNA topoisomerase
Prevents tangling (supercoiling) of DNA ahead of the replication fork.
DNA helicase
Unwinds DNA at the replication fork
DNA polymerase forms…
a phosphodiester bond to link DNA nucleotides together
What catalyzes the formation of a phosphodiester bond between the incoming nucleotide and the previous nucleotide
DNA polymerase