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Phlebotomy: colors, additives, uses, operations

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Total cards: 24•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (24)

#1
Front (Question)

Explain the mnemonic: Stop-Light-Red Stay-Put Green-Light-Go

Back (Answer)

• Stop – Sterile (yellow) • Light – Light blue tube • Red – Red top tube • Green – GTT (Green top tube) • Light – LTT (Lavender top tube) • Go – (Gray-top tube) • Stay – SST/Red gray (Serum separated tube) • Put – PST/Lt green (Plasma separated tube)

#2
Front (Question)

• ) Three methods of drawing blood • ) Capjet use method for infants

Back (Answer)

Three methods of blood draw • Vacutainer method/needle • Butterfly method/syringe-needle • Fingerstick/milking Capjet method for infants • Heel stick, collect a drop, mix, repeat

#3
Front (Question)

• With an MI, collect a blood sample and _____. • One should always draw above or below the IV line?

Back (Answer)

• Centrifuge, do not wait for it to clot • Below

#4
Front (Question)

Additives in yellow-top tubes and their function

Back (Answer)

Sodium polyethanol sulfate (SPS) Blood culture specimen collection in microbiology Acid citrate dextrose (ACD) A or B Blood bank studies HLA phenotyping DNA Paternity testing

#5
Front (Question)

Additive in blue-top tubes

Back (Answer)

• Buffered sodium citrate • Citrate, theophylline, adenosine dipyridamole (CTAD)

#6
Front (Question)

Laboratory use of light blue tubes

Back (Answer)

• Routine coagulation determination • CTAD for platelet function assays

#7
Front (Question)

What is the additive in red-top tubes? What is the laboratory use of red-top tubes?

Back (Answer)

Clot activator (plastic siding) Laboratory use (60 sec to clot): • Chemistry serum determination • Blood donor screening • Infectious disease screening

#8
Front (Question)

• Orange tube additive • Laboratory uses of orange-top tubes

Back (Answer)

• Thrombin clot activator (5 minutes) • Chemistry stat serum determinations

#9
Front (Question)

Additives in gold or red/gray top tubes Laboratory use of red/gray top tubes

Back (Answer)

• Clot activator • Gel for serum separation • Chemistry serum determination • Routine blood donor screening • Diagnostic testing for infectious disease • Clotting time = 30 minutes

#10
Front (Question)

Light green/gray-tube • Additives • Laboratory Use

Back (Answer)

Additives • Lithium heparin • Gel for plasma separation Laboratory use • Chemistry plasma determination

#11
Front (Question)

Green top tube additives

Back (Answer)

Sodium heparin Lithium heparin

#12
Front (Question)

Green top tube laboratory use

Back (Answer)

Chemistry plasma determination

#13
Front (Question)

Lavender top tube additives (2)

Back (Answer)

Liquid K3 EDTA K2 EDTA

#14
Front (Question)

Pink top tube additives

Back (Answer)

K2 EDTA

#15
Front (Question)

White top tube additives and laboratory use

Back (Answer)

K2 EDTA and gel for plasma separation • Molecular diagnostic test methods: • PCR and/or branched DNA (bDNA)

#16
Front (Question)

Lavender and pink top tube laboratory use

Back (Answer)

Whole blood hematology determination K2 EDTA used for • Immunohematology testing • Blood donor screening

#17
Front (Question)

Gray top tube additives

Back (Answer)

• Sodium flouride + Potassium oxalate • Sodium flouride + Na2 EDTA • Sodium fluoride

#18
Front (Question)

Gray top tube laboratory use

Back (Answer)

Glucose determination • Oxalate and EDTA will give plasma samples • Sodium flouride is the antiglycolytic agent

#19
Front (Question)

Royal blue additives

Back (Answer)

Clot Activator K2 EDTA

#20
Front (Question)

Royal blue laboratory use

Back (Answer)

• Nutritional chemistry determinations • Trace elements • Toxicology

#21
Front (Question)

Tan top tube additives

Back (Answer)

K2 EDTA

#22
Front (Question)

Tan top tube laboratory use

Back (Answer)

Lead determination Certified to contain less than .01 ug/mL (ppm) lead

#23
Front (Question)

Clear red/gray top tube additives Clear red/gray top tube laboratory use

Back (Answer)

None Discard or secondary specimen

#24
Front (Question)

Tubes of particular importance

Back (Answer)

Red (general, bloodbank) Light Blue (coagulation) Lavender (diff, cbc)