Quiz 2

Fluorescence Recovery After Photobleaching. Molecular cloning, photobleaching an area that removes fluorescence. Rate of protein movement studied by how fast the proteins restore fluorescence.

Fluorescence Resonance Energy Transfer 2 Different proteins undergo molecular coding with tracers next to each other on color wheel. Fluorescent light excitations will emit a color 2 off on the wheel if proteins are interacting. Determines interactions between each protein.

best res, electron beam for energy, fixed cells only, electron detector and screen used. specimen receives metal salts stain and beam collects salts.

Scanning Electron Microscopy, creates image from detecting reflected or knocked off electrons

Transmission Electron Microscopy, observes the electrons transmitting through the sample

Sonication - sound is used to break PM Detergent used to make holes in PM Pushing cell through small hole at high pressure to tear open PM Homogenization - grinder or blender breaks PM, mortar and pestle

1st Nuclei 2nd Mitochondria, lysosomes, peroxisomes 3rd PM and ER 4th Ribosomes

unlimited capability, fertilized egg

gives rise to some but not all cells

gives rise to cells with a particular function

Multipotent, found in bone marrow that produce lymphoid and myeloid progenitors

B, T, NK, lymphoid dendritic

macrophages, eosinophil, neutrophil, basophil, platelets, myeloid dendritic, erythrocytes

intestines are lined with wavy sheets of cells, peaks villus, bottoms crypt. bottom has multipotent SCs that make 3 types of cells

Goblet, Absorptive Epithelial, Enteroendocrine cells

Multipotent SCs, transit amplifying cell, differentiated into one of the three.