Concepts In Instrumental Methods

follows the Beer’s law but only uses filters as monochromator

light absorbed indicates concentration with the use of deuterium lamp

includes filters, prisms and diffraction gratings

best method to separate uncharged carbohydrates/sugars of same number of carbons

best method to separate charged amino acids by adjusting pH to match isoelectric point

the principle involved in the measurement of glucose involving the use of reagent strips

includes tungsten filament lamp, xenon lamp, hydrogen lamp, and hollow cathode lamp

includes photomultiplier tubes, barrier layer cells, and photodiode arrays

technique used primarily in therapeutic drug monitoring and fetal lung maturity testing

technique which does not require light source and employs coenzyme-luciferase system

principle involved in IS measurement of electrolytes and PCO2 determination

includes computer printers, digital windows, and galvanometer or micrometer scales

includes PO measurement via the Clark electrode and Cotlov chloride titrator techniques

technique that is sensitive to contaminants leading to quenching; 2 types of spectra used

method which uses hollow cathode lamp and uses flame as cuvet and as agent to convert ions to ground state