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Cholexterol Determination

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Total cards: 24•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (24)

#1
Front (Question)

Cholesterol Esterase (CE) ________ esters to free cholesterol and fatty acids

Back (Answer)

hydrolyzes

#2
Front (Question)

wavelength?

Back (Answer)

500 nm

#3
Front (Question)

Reagent and Standard are stable stored ________ until expiration date on label

Back (Answer)

2-8 degrees celcius

#4
Front (Question)

what temperature must a medtech bring the reagent and standard before use

Back (Answer)

Room Temperature

#5
Front (Question)

Blood should be collected following a _________ specimen

Back (Answer)

12 hour fast

#6
Front (Question)

What should be avoided in collecting sample

Back (Answer)

hemolysis

#7
Front (Question)

cholesterol standard

Back (Answer)

200mg/dL

#8
Front (Question)

What color is the intensity proportional to total cholesterol concentration?

Back (Answer)

Red color or O-Quinoneimine dye

#9
Front (Question)

Incubate all cuvettes at ______ for _____ minutes

Back (Answer)

37 degree celcius, 5 minutes

#10
Front (Question)

How many mL/uL Standard- Standard (s)

Back (Answer)

0.01 mL (10uL)

#11
Front (Question)

Reagent: RB, S, U

Back (Answer)

1.0mL

#12
Front (Question)

Sample to Sample (U)

Back (Answer)

0.01 mL (10uL)

#13
Front (Question)

Read S and U vs RB at ____ nm within ___ minutes

Back (Answer)

500 nm, 60 minutes

#14
Front (Question)

Reference Range: Adolescent

Back (Answer)

120-210 mg/dL

#15
Front (Question)

Reference Range: Adult

Back (Answer)

140-310 mg/dL

#16
Front (Question)

Reference Range: Desirable for Adult

Back (Answer)

140-200 mg/dL

#17
Front (Question)

Reference Range: Newborn

Back (Answer)

53-135 mg/dL

#18
Front (Question)

Reference Range: Infant

Back (Answer)

70-175 mg/dL

#19
Front (Question)

Reference Range: Child

Back (Answer)

120-200 mg/dL

#20
Front (Question)

_______________ with absorption maxima at 500 nm is produced when phenol is oxidatively coupled with ______________ in the presence of ______________ with ___________

Back (Answer)

Quinoneimine Chromogen 4-aminophenazone Peroxidase Hydrogen Peroxide

#21
Front (Question)

How many months can extend the stabilities at -20C for Total Cholesterol?

Back (Answer)

3 months

#22
Front (Question)

How many days can extend the stabilities at -20C for HDL Cholesterol?

Back (Answer)

7-14 days

#23
Front (Question)

Interfering substance: Anticoagulants such as ______________ and _____________ will result in false low values

Back (Answer)

sodium flouride and oxalate

#24
Front (Question)

Cholesterol Reagent Components

Back (Answer)

4-aminophenazone Phenol Peroxidase Cholesterol Esterase Cholesterol Oxidase Buffers and Stabilizers