GGT

gamma-glutamyltranspeptidase

Liver and bile duct

Hepatic or biliary origin

Together with other enzymes such as alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and cholinesterase gamma-GT

Kinetic photometric test according to Szasz/Persijn

Gamma-GT catalyzes the transfer of glutamic acid to acceptors like glycylglycine in this case. This process releases 5-amino-2-nitrobenzoate which can be measured at 405 nm. The increase in absorbance at this wavelength is directly related to the activity of gamma-GT.

L-Gamma-glutamyl-3-carboxy-4-nitranilide + Glycylglycine < Gamma-GT > Gamma-glutamyl-glycylglycine + 5-Amino-2-nitrobenzoate

TRIS ph 8.28 (135 mmol/L) Glycylglycine 135 mmol/L

L-Gamma-glutamyl-3- carboxy-4-nitroanilide ph 6.00 (22 mmol/L)

• The reagents contain sodium azide (0.95 g/L) as preservative. Do not swallow! Avoid contact with skin and mucous membranes. • In very rare cases, samples of patients with gammopathy might give falsified results [8]. • Please refer to the safety data sheets and take the necessary precautions for the use of laboratory reagents. For diagnostic purposes, the results should always be assessed with the patient’s medical history, clinical examinations and other findings. • For professional use only!

405 nm

1cm

37 degrees

Against reagent blank

Szasz. IFCC 1421. 1606 1158. 1309

Women < 32 (0.53 ukat/L) Menz. <49U/L (0.82 ukat/L)

Female <38 U/L (0.63 ukat/L) Male <55 U/L (0.92 ukat/L)