Genetic standardisation
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What are outbred lines also called?
stocks
What are inbred lines also called?
strains
Name some characteristics of outbred stocks?
• heterozygosity undefined • genetic profile unkown • random-mated, close colonies • high reproductive performance and high vigor • phenotypically variable • not identifiable - not defined by genetic profile • cheap and large resemble human population
How are inbred strains defined?
The result of more than 20 consecutive generations of brother-sister mating An inbred strain may be regarded as an immortal clone of an almost constant genotype
Name some properties of inbred strains
• isogenicity • homozygosity • phenotypic uniformity • long term stability • individuality of the strain • internatl distribution • identificaition (PCR primers available) • senstivity (to, e.g., environmental factors)
When should outbred stocks be used in research?
• large amounts of tissue • education • base population for selective breeding • genetic mapping
When should inbred strains be used?
In all cases where use of outbred stocks is not particularly justified
What are F1 hybrids?
• Cross between 2 inbred strains • heterozygosity high • isogenic • robust - high vigour
Name main causes for genetic change
• genetic contamination or immigration • mutation • directional selection • random drift