Chloride

Photometric test using ferric (III) perchlorate

Chloride forms with ferric ions a yellow colored complex whose absorption is measured at 340 nm. A discoloring agent in reagent 2 displaces Chloride out of the complex, thereby discoloring the solution. The difference in absorbance between the colored and discolored state of the solution is proportional to the concentration of chloride in the sample.

R1: Methanesulfonic acid Ferric (III) perchlorate R2: Inorganic salt

Serum or plasma (lithium heparin) Separate from cellular contents immediately after blood collection.

340/660 nm (bichromatic)

3.545

95-105 mmol/L