Ex 3:Aseptic Transfer Techniques
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What are aseptic/sterile techniques?
procedures done to protect the culture, worker, and environment from contamination
What is a pure culture?
culture free of contamination and containing organisms descended from one original cell
Why are pure cultures important?
for identifying bacteria with biochemical tests, performing antibiotic sensitivity testing or maintaining stock cultures
What is a pathogen?
disease producing micro-organism
Why do microbiologists need to transfer bacteria?
bacteria need fresh nutrients, be removed from their own toxic waste products, or when biochemical testing of an isolated species is necessary
What is flaming?
sterilizing loops/needles by holding wire into a flame until red hot
What are aerosols?
droplets of micro-organisms
What is a bacticinerator?
special oven that sterilizes needles/loops, culture tubes. consists of a ceramic funnel tube
What is a pipet used for?
transfer of liquids including sterile culture mediums, bacterial culture or reagents
What is alcohol flaming?
dangerous sterilization technique that sterilizes forceps by dipping them into alcohol and passing through a flame