ALP
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Alkaline phosphatase originate mainly from
Bone and liver
ALP is also from other tissues such as
Kidney, placenta, testes, thymus, lung and tumors
Physiological increases are found during
Bone growth in childhood Pregnancy
Pathological increases are largely associated with
Hepatobiliary and bone diseases
In hepatobiliary disease they indicate obstruction of the bile ducts as in ______ caused by
Cholestasis Caused by: gall stones, tumors or inflammation
Elevated activities are also observed in
Infectious hepatitis
In bone diseases elevated AP activities originate from increased osteoblastic activity as in
Paget’s disease, osteomalacia (rickets), bone metastases and hyperparathyroidism.
Method
Kinetic photometric test, according to the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC)
Components and concentrations of Reagent 1
2-Amino-2-methyl-1-propanol Magnesium acetate Zinc sulphate HEDTA
Components and concentrations of Reagent 2
p-Nitrophenylphosphate
Sample Start
Mix 4 parts of R1 + 1 part of R2
Stability of the reagent
4 weeks at 2 – 8°C 5 days at 15 – 25°C
Specimen
Serum or heparin plasma
Wavelength
Hg 405 nm, 400 – 420 nm
Optical path
1 cm
Temperature
37°C
Measurement
Against reagent blank
Conversion Factor
0.0167 = AP [µkat/L]
recommended for calibration.
DiaSys TruCal U
Measuring range up to
1400 U/L.
Limit of detection
0.6 U/L
Onboard stability
6 days
Calibration stability
6 days
Interfering substance
Ascorbic acid Bilirubin (conjugated) Bilirubin (unconjugated) Hemoglobin Lipemia (triglycerides)