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Immunochemical Techniques

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Total cards: 24•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (24)

#1
Front (Question)

Competitive formats use ________ reagents (antibody or other antigen binder)

Back (Answer)

LIMITED

#2
Front (Question)

Noncompetitive formats use _______ reagents (antibody or other antigen binder) and are two-site or “________” assays

Back (Answer)

EXCESS sandwich

#3
Front (Question)

What does it mean that competitive immunoassays are simultaneous?

Back (Answer)

all reactants are mixed together simultaneously or at the same time

#4
Front (Question)

Simultaneous Competitive Immunoassay: - Principle

Back (Answer)

Labled and unlabeled antigen compete for a limited number of binding sites on an antibody binder. Avidity for the antibody is the same for both.

#5
Front (Question)

Simultaneous Competitive Immunoassay: - Proportionality observed between bound able and unlabeled antigen concentrations

Back (Answer)

The probability of labeled Ag binding to the antibody binder is inversely proportional to (unlabeled Ag)

#6
Front (Question)

Simultaneous Competitive Immunoassays - Examples of tests

Back (Answer)

RIA, FIA, EIA

#7
Front (Question)

Noncompetitive Immunoassay: - Principle

Back (Answer)

A captured antibody is bound to a solid phase. Unlabeled antigen reacts with the solid phase antibody, then there’s a washing step. Labeled antibody is added; it reacts with the unbound antigen on a 2nd and distinct epitope. Another washing step occurs, and the amount of the bound label is determined

#8
Front (Question)

Noncompetitive Immunoassay: - Proportionality observed between bound able and unlabeled antigen concentrations

Back (Answer)

directly proportional

#9
Front (Question)

What is the difference of a heterogeneous immunoassays from homogeneous immunoassays?

Back (Answer)

Heterogeneous require a separation step to separate the bound label from the free, unbound reactants. Homogeneous do not require a separation step

#10
Front (Question)

EMIT: - what does it stand for

Back (Answer)

Enzyme-Multiplied Immunoassay Technique

#11
Front (Question)

EMIT: - What is detected

Back (Answer)

change in absorbance after adding substrate

#12
Front (Question)

EMIT: - Homogeneous or heterogeneous

Back (Answer)

Homogenous

#13
Front (Question)

EMIT: - Proportionality

Back (Answer)

direct

#14
Front (Question)

ELISA: - what does it stand for

Back (Answer)

Enzyme-Linked Immunosorbent Assay

#15
Front (Question)

ELISA: - what is detected

Back (Answer)

Change in absorbance

#16
Front (Question)

ELISA: - Homogeneous or heterogeneous

Back (Answer)

Heterogeneous

#17
Front (Question)

ELISA: - Proportionality

Back (Answer)

Direct or indirect

#18
Front (Question)

MEIA: - What does it stand for

Back (Answer)

Microparticle Enzyme Immunoassay

#19
Front (Question)

MEIA: - What is detected

Back (Answer)

fluorescences

#20
Front (Question)

MEIA: - Homogeneous or Heterogenous

Back (Answer)

Heterogenous?

#21
Front (Question)

MEIA: - Proportionality

Back (Answer)

Direct

#22
Front (Question)

FIA: - What does it stand for

Back (Answer)

Fluorescent Immunoassays

#23
Front (Question)

FIA: - What is detected

Back (Answer)

Fluorescence

#24
Front (Question)

FIA: - Homogeneous or Heterogeneous

Back (Answer)

Heterogeneous