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Genetics lab midterm

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Total cards: 10•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (10)

#1
Front (Question)

SNP lab background

Back (Answer)

PTC is bitter taste; Recessive allele are unable to taste PTC

#2
Front (Question)

TAS2R38 gene

Back (Answer)

encodes bitter taste receptor; contains three single nucleotides polymorphism

#3
Front (Question)

DNA isolation (Monarch Spin gDNA)

Back (Answer)

DNA suitable for PCR

#4
Front (Question)

Lysis used?

Back (Answer)

Cell lysis buffer: mild lysis conditions to reduce viscosity. optimized with Proteinase K

#5
Front (Question)

Chaotropic salt-based binding buffer

Back (Answer)

Low alcohol content; Allows specific binding of gDNA with minimal RNA binding; low speed

#6
Front (Question)

RNase A

Back (Answer)

We use low alcohol cause so biding of gDNA alone

#7
Front (Question)

Steps

Back (Answer)

Disrupt and Lyse sample; Bind DNA to matrix; Wash matrix; Elute DNA

#8
Front (Question)

DNA purity

Back (Answer)

A260/A280

#9
Front (Question)

DNA integrity

Back (Answer)

Gel electrophoresis

#10
Front (Question)

Back (Answer)