Module 3d: Chromatography

Refers to the group of techniques used to separate complex mixtures on the basis of different physical and chemical interactions between the compounds and the stationary phase.

Four basic components in any chromatographic technique:

Component that carries the complex mixture (sample)

component which can either be solid or liquid through which the mobile phase flows

component that holds the stationary phase

The separated components

5 separation mechanisms of chromatography

Separation mechanism that is based on the differences or magnitude of their ionic charge.

An exchangable ion found in close proximity with the fixed charge to maintain electrochemical neutrality.

Ion exchange chromatography used primarily for strongly acidic groups

Ion exchange chromatography used primarily for strongly basic quarternary amines with positive charges.

Chromatography involving separation of solutes based on their differential distribution of between two immiscible liquids

partition chromatography separates solutes based on

Partition chromatography is classified into

Liquid liquid chromatography is further classified into:

Normal phase LLC has

Reverse phase LLC has

Version of partition chromatography wherein analytes elute in order of increasing polarity

Separation technique based on the differences between the adsorption and desorption of solutes at the surface of a solid.

adsorption chromatography is also known as

In adsorption chromatography, the molecules that are most soluble in the mobile phase move the ___________

Separation method based on the molecular sizes and shape

In size exclusion chromatography, the molecules that remain entirely in the mobile phase are large or small??

Process where hydrophilic gel isused in separation of enzymes, antibodies and proteins.