Ch 34 Miscellaneous Separation Method

The physical state of a substance held/ heated above its critical temperature

Is the temperature above which a substance cannot be liquified

Acts as the mobile phase in supercritical fluid chromatography and are able to dissolve large nonvolatile molecules

The density of a supercritical fluid is ___ to ____ times that of its gaseous state

The pumping system in SFC that keeps the fluid in liquid state

The effects of pressure to the density of the supercritical fluids

The most widely used mobile phase for supercritical fluid chromatography

Give the planar chromatographic techniques

Named the different detectors than can be used in SFC

Can be considered a form of liquid-solid chrom in which the stationary phase is a thin layer on the surface of a plate. The mobile phase is drawn by capillary action

This is a chromatographic technique where the mobile phase moves through the stationary phase by capillary action, sometimes assisted by gravity or an electrical potential

Commercial plates used in TLC that have thicker layers of 200 to 250 μm, particle size of >20 μm

Commercial plates used in TLC that have thicker layers of 100 μm, particle size of <5 μm

The most critical aspect of TLC where the sample is applied as a spot 1 to 2 cm from the edge of the plate

Increases the precision and accuracy of sample application

The process by which a sample is carried through the stationary phase by a mobile phase. Analogous to elution in liquid chromatography

The process of locating analytes on a thin-layer plate is often termed as

Two common methods in locating the analytes on the plate

Samples are placed on both ends of the plate and developed towards the middle, thus doubling the number of samples

Two types of developing chamber

Separation of analytes that uses a stationary phase from a highly purified cellulose with close control over porosity or thickness

The separation method based on the differential rates of migration of charged species in an applied electric field

Have a unique ability to separate charged molecules of interest

An electrophoresis that is carried out in columns and separate micro amounts of sample in fused-silica capillary tubes