Semester 2 Pracs
暂无描述。系统推荐的高质量记忆内容，适合每天坚持背诵学习。
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Why do amino acids move high up the TLC plate
Since the solvent is less polar than the stationary phase, the non-polar amino acids will move higher up the TLC plate
Formula for Rf values
Distance of sample from origin/Distance between origin and solvent front
How can you determine components of sample mixture
Location of dots compared to other
How were the amino acids in the mixture able to be separated using TLC
Separated based on affinity for stationary phase, polar TLC plate, and mobile phase, the non-polar solvent. Non polar substances had higher affinity for mobile phase leading to a higher rise. Polar substances climbed less as they formed dipole-dipole interactions with the stationary phase, which need more energy to break, and therefore stay in there longer and don’t move up
How does HPLC differ from TLC
HPLC’s stationary phase has smaller and more absorbent particles which makes the separation more evident. HPLC mobile phase is forced through a column packed with stationary phase under high pressure
Time taken for: HPLC, TLC
Fast Fast
Sample size of: HPLC, TLC
Small to moderate Very small
Detection method: HPLC, TLC
Automated UV Light, eyes
Advantages: HPLC, TLC
High sensitivity, qualitative Simple
Disadvantages: HPLC, TLC
Expensive, need training Qualitative, manual errors
What formula do you use to find pressure of butane
P(atm) = P(but) + P(H2O)
Solubility of nitrates
Soluble
Solubility of chlorides
Generally soluble except silver, lead
Solubility of sulphates
Generally soluble except silver, lead, barium, calcium, strontium
Solubility of carbonates
Generally not soluble except potassium, sodium, annomium
Observations for HCL + blue litmus and HCL + red litmus
Red litmus paper stayed red Blue litmus paper turned red
Observations for NaOH + blue litmus and NaOH + red litmus
Red litmus paper turned blue Blue litmus paper stayed blue
Which metals give a pop when reacted with HCl? Mg, Zn, Al and Cu
Mg, Zn, Al
Observation for reaction of HCl with either Mg, Zn or Al
Colourless solution added to metal grey solid bubbles produced and colourless solution forms
Observation for reaction of HCl with Cu
Colourless solution added to salmon pink solid. No change
Which metals give a pop when reacted with NaOH? Mg, Zn, Al and Cu
Zn, Al
Observation for reaction of NaOH with either Mg or Cu
Colourless solution added to metal grey/salmon pink solid. No change
Observation for reaction of NaOH with either Zn or Al
Colourless solution added to metal grey solid. Bubbles produced, solution remains colourless
Observation for reaction between CuO and HCl
Black solid added to colourless solution to produce rapid bubbling and solution turns green