Pipetting Technique and Centrifugation (P)

A technique used to transfer aliquot of liquid sample from one container to another

1) According to Design 2) According to Drainage 3) According to Type

1) To contain (TC) 2) To deliver (TD)

TC pipette holds or contains a particular volume but does not dispense that exact volume

TD will dispense the volume indicated

TD pipettes are more common than TC pipettes

1) Blow-out 2) Self-draining

Blow-out pipette has frosted bands or two continuous rings or etched rings at the top

The last drop is fully expelled into the receiving vessel

Aspirator bulb

The contents of self-draining pipette are drained by gravity

Measuring or Graduated

Used to transfer reagents and make dilutions Used to dispense several volumes

1) Serologic 2) Mohr 3) Micropipette

1) The graduations are located after the tip of the pipette 2) It is a blow-out pipette

1) The graduations are located before the tip of the pipette 2) It is a self-draining pipette

< 1 mL

Micropipettes can be designed as either serologic or Mohr pipette

Micropipettes are the small version of measuring pipettes

Yes, including the dilutions or major divisions where the specific pipette can be used

1) Volumetric 2) Ostwald-Folin 3) Pasteur pipettes 4) Automatic pipettes

Transfer pipette

It dispenses one volume without further subdivisions

1) It carries aqueous solutions 2) Most accurate and precise 3) Self-draining