Exam 2
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RNA synthesis
Transcription
Protein synthesis
Translation
_____ can be expressed with different efficiencies
Genes
Difference between DNA and RNA?
Deoxyribose vs Ribose Thymine vs Uracil Double Stranded vs Single Stranded
DNA transcription produces a single-stranded RNA that is _____________ to one strand of DNA
complimentary
DNA is transcribed by the enzyme ___ __________ in bacteria
RNA polymerase
RNA is synthesized in the __ to __ direction
5’ to 3’
mRNA
Messenger RNA, codes for proteins
rRNA
Ribosomal RNA, form the basic structure of the ribosome and catalyze protein synthesis
tRNA
transfer RNA, central to protein synthesis as adaptors between mRNA and amino acids
TBP
TATA Binding Protein
____________ _______ in DNA causes DNA supercoiling
Superhelical tension
DNA with free end —> one helical turn —>
DNA helix must rotate one turn
DNA with fixed ends —> one helical turn —>
DNA helix forms one supercoil
Negative supercoiling
Helix opening facilitated
Positive supercoiling
Helix opening hindered
Every molecule of RNA synthesized by RNA polymerase II is capped at the __ end
5’
Eukaryotic polymerase II acts like an
RNA factory
In eukaryotes, RNA is spliced _____ transcription
after
To produce different proteins from the same DNA sequence, some genes have _________ ________ _______
alternate splicing schemes
The 3’ end of eukaryotic mRNA is processed so that a ____________ _________ associated with specific binding proteins is formed.
poly-adenine structure
mRNA is transported out of the nucleus through the
nuclear pore complex
rRNA precursors are processed into _________ in the _________
ribosomes, nucleolus
Redundancy in the genetic code?
Multiple codes for one amino acid