EXAM III Proteins, COH, Lipids
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Normal glucose concentration of serum?
80-100 mg/dL
Requirements of an oral GTT
Fasting at least 10 hours Done in morning no eating, drinking, smoking or exercise normal to high COH intake 3 days prior
Order of mobility of serum proteins during an electrophoresis
albumin(fastest)-a1-a2-beta-gamma
Why can the biuret method NOT be used for determining CSF or urine protein?
it lacks sensitivity to detect low levels of protein which are in those specimens
What does the biuret reagents specifically react with?
tripeptide 2 or more peptide chains
What is the reference method for total protein determination?
Kjeldahl
Which method for measuring albumin is most common in automated instruments?
Dye-binding
What SPE abnormality is seen in multiple myeloma? In nephrotic syndrome?
Spike in gamma region ^ a2 and beta region, decrease albumin
Glucose in CSF is approximately ____ of the amount of glucose in the blood.
2/3 60-70%
In bacterial meningitis, what would be the expected results for CSF protein? CSF glucose?
protein increased glucose decreased
What anticoagulant is used to prevent glycolysis?
sodium fluoride
Increases blood glucose levels inhibits glucose uptake by the tissues increases glucose output by the liver
ACTH growth hormone
Increases blood glucose levels Increases glycogenolysis and gluconeogenesis
Glucagon
Increases blood glucose levels Increasing gluconeogenesis and inhibiting glucose metabolism in peripheral tissue
Glucocorticoids
Decreases blood glucose levels by decreasing glycogenolysis-only hormone that decreases
Insulin
Increases blood glucose levels fight or flight stimulates glycogenolysis
Epinephrine
What is the action of the enzyme glucose-6-phosphate dehydrogenase?
removes hydrogen from glucose-6-phosphate and gives it to NADP
What technique is used to ID patients with familial hyperlipoproteinemia?
electrphoresis
normal value for albumin
3.5-5.0 g/dL
normal value for globulin
2.5-3.5 g/dL
normal value for TP
6.0-8.5 g/dL
What phenom causes the gamma globulins to move toward the cathode in SPE, even though they are negatively charged?
EEO electroendosmosis
What specific form of hgb is used to monitor patients with Type I diabetes mellitus?
A1c
How is globulin in serum routinely determined?
TP - albumin