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Module 10

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Total cards: 19•Content version: v3•Public deck•Updated: 8/1/2026

Card preview (19)

#1
Front (Question)

Which end does transcription of the coding strand start?

Back (Answer)

the 5’ end

#2
Front (Question)

What is the enzyme used for transcription of DNA strand to mRNA?

Back (Answer)

RNA polymerase II

#3
Front (Question)

Which sub unit of the Ribosome is responsible for transcription?

Back (Answer)

RNA polymerase

#4
Front (Question)

How do you initiate the transcription on the DNA strand

Back (Answer)

Promoter

#5
Front (Question)

What is the use of the TATA box?

Back (Answer)

It is a consensus site for the promoter

#6
Front (Question)

What is the use of TBP?

Back (Answer)

General transcription factor

#7
Front (Question)

Where does the incoming NTP bind to?

Back (Answer)

The carboxyl group of the previous base

#8
Front (Question)

What is RNA polymerase I transcripting

Back (Answer)

rRNA

#9
Front (Question)

What is RNA polymerase III transcripting?

Back (Answer)

tRNA and rRNA

#10
Front (Question)

What is the shape of mitochondria genome, is there introns?

Back (Answer)

Circular and there are no intron in eukaryotic cell

#11
Front (Question)

Is genetic code of mitochondria the same the DNA in the nuclear?

Back (Answer)

No

#12
Front (Question)

Does mitochondria create its good tRNA?

Back (Answer)

yes

#13
Front (Question)

What causes disease in mitochondria?

Back (Answer)

Mutation in the mitochondrial genome or mitochondrial genes in the nuclear genome

#14
Front (Question)

How do you find out the mRNA in a gene?

Back (Answer)

RT-PCR

#15
Front (Question)

How do you remove DNA in RT-PCR?

Back (Answer)

DNase 1

#16
Front (Question)

How do you find the mRNA in the solution?

Back (Answer)

using synthetic oligonucleotide (oligo-dT) primer, prime in the poly AAA tail

#17
Front (Question)

What is semi-quantitative RT-PCR experiment for?

Back (Answer)

To find the how highly expressed genes there are in the genome of the cell

#18
Front (Question)

How do you find the what proteins there are in a gene?

Back (Answer)

Using SDS PAGE

#19
Front (Question)

What is used to recognise proteins

Back (Answer)

Using antibodies in a western blot