KIDNEY FUNCTION TEST part 2

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Total cards: 24•Content version: v4•Public deck•Updated: 8/1/2026

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Front (Question)

This refers to the concentration of urea as a whole molecule not just by the nitrogen portion.

Back (Answer)

Urea concentration test

Front (Question)

The values of Urea is often obtained via?

Back (Answer)

Direct methods

Front (Question)

What is the equation that is used to convert BUN in the equation?

Back (Answer)

Urea concentration = BUN x 2.14

Front (Question)

How is 2.14 obtained?

Back (Answer)

It is derived from: - molecular weight of 2 nitrogen atoms in urea.

Front (Question)

The stability of Urea in Serum can lasts up to?

Back (Answer)

24hrs at room temp several days: 2 - 6°C 2 - 3 months when frozen

Front (Question)

These substances inhibits the urease enzyme

Back (Answer)

Fluoride and Citrate

Front (Question)

This is contradicted in the assay

Back (Answer)

Double oxalate

Front (Question)

If urea in serum is left standing for a prolonged period, what would happen?

Back (Answer)

Due to deamination, the NH3 concentration would increase 2-3 times.

Front (Question)

Do lipemia and hemolysis interfere with urea concentration test?

Back (Answer)

No.

#10

Front (Question)

TRUE OR FALSE: Ammonium containing anticoagulant should not be used in urea concentration test.

Back (Answer)

TRUE

#11

Front (Question)

TRUE OR FALSE: In Urea concentration test, fasting sample is required.

Back (Answer)

FALSE; Not required

#12

Front (Question)

This method measure urea by converting it first to ammonium ions using urease.

Back (Answer)

Indirect method / Enzymatic

#13

Front (Question)

What happens in the initial reaction of indirect/ enzymatic method?

Back (Answer)

The urease converts urea to ammonium ions and carbonate ions/ carbon dioxide

#14

Front (Question)

What happen in the secondary reaction of indirect/ enzymatic method?

Back (Answer)

It quantifies ammonium ions that formed after the initial reaction

#15

Front (Question)

Where in urease often derived?

Back (Answer)

Jack bean meal

#16

Front (Question)

What is the color dimercuric ammonium iodide.

Back (Answer)

Yellow-orange

#17

Front (Question)

This is the conversion of unionized urea into ammonium and bicarbonate and it measures the increase in conductivity rate.

Back (Answer)

Beckman BUN analyzer

#18

Front (Question)

Beckman BUN analyzer is based from?

Back (Answer)

Conductivity generated when NH4 ions and HCO3 are produced from the reaction of urease on urea.

#19

Front (Question)

The Beckman BUN analyzer in monitored by?

Back (Answer)

Conductivity electrode

#20

Front (Question)

Urastrat Strip Method is based on?

Back (Answer)

Chromatography

#21

Front (Question)

What travels up the urastrat strip?

Back (Answer)

Serum or plasma

#22

Front (Question)

What are the reactants involved in Urastrat Strip Method?

Back (Answer)

Urease K2CO3 BGC-Tartaric acid

#23

Front (Question)

What is the formula of BUN mg/dL every 30 minutes?

Back (Answer)

Height of color change (mm) x 5 + 10

#24

Front (Question)

What is the reference method for DAM?

Back (Answer)

Fearon