Amino acids, Proteins & DNA: QP
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Suitable developing agent in TLC and why it is needed [2]
[1] ninhydrin [2] amino acids are colourless / to make the amino acids visible
Explain why the strength of the interaction between two cysteine R groups differs from the strength of the interaction between a serine R group and an aspartic acid R group. Use the Data Booklet to help you answer this question. [4]
[1] Two Cys R groups form a disulfide bridge/link stated or described [2] Ser and Asp R groups form Hydrogen bonds [3] Disulfide bridges are stronger than H bonds [4] Because disulfide bridges are covalent bonds (while H bonds aren’t)
What is an amide group [1]
[1] C=O attatched to N-H
Why is it necessary sometimes to use two different solvents [1]
[1] Some of the amino acids did not separate/dissolve with the first/either solvent OR Some amino acids have the same Rf value or have the same affinity with the first/either solvent
State the meaning of the term complementary when it is used to refer to DNA strands [1]
[1] A to T and C to G