Analytical Methods and Instrumentation
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____ provide the foundation for all measurements made in a modern clinical chemistry laboratory.
Analytic Techniques and Instrumentation
Four Basic Disciplines
• Spectrometry • Luminescence • Electroanalytic Methods • Chromatography
Methods under Spectrometry
• Spectrophotometry • Atomic Absoption Spectrometry (AAS) • Mass Spectrometry (MS)
Methods under Luminescence
• Fluorescence • Chemiluminescence
Methods under Electroanalytic Methods
• Electrophoresis • Potentiometry/Amperometry
Methods under Chromatography
• Gas • Liquid • Thin-layer
Other Analytical Methods
• Colorimetry • Volumetric (Titrimetric) • Turbidimetry • Nephelometry • Fluorometry • Osmometry • Electrochemistry Techniques
The primary goal of ____ is the isolation of discreet portions of the sprectrum.
Photocolorimetry
Photocolorimetry is used for measuring ____ in the laboratory.
analytes
Measurements under Photocolorimetry
• Spectrophotometric measurement • Photometric measurement
____ is the measurement of light intensity in a narrower wavelength.
Spectrophotometric measurement
____ is the measurement of light intensity without consideration of the wavelength.
Photometric measurement
____ involves the measurement of the light transmitted by a solution to determine the concentration of the light-absorbing substance.
Spectrophotometry
Two Types of Spectrophotometer
• Single Beam • Double Beam
____ is the simplest type of spectrophotometer.
Single Beam Spectrophotometer
____ is designed to make one measurement at a time at one specified wavelength.
Single Beam Spectrophotometer
In ____, the absorpition maximum of the analyte must be known in advanced.
Single Beam Photometer
In single beam spectrophotometer, the ____ must be known in advanced.
maximum absorption
____ splits the monochromatic light into two components.
Double Beam Spectrophotometer
In ____, one beam passes throughout the sample and the other beam passes through reference solution or reagent blank.
Double Beam Spectrophotomer
What is the purpose of the additional beam in Double Beam Spectrophotometer?
To correct for variation of light source intensity
In double beam spectrophotometry, absorbance of the sample can be recorded directly as the ____ of the sample beam.
electrical output
2 Types of Double Beam Spectrophotometer
• Double-beam in space • Double-beam in time
____ uses two photodetectors for the sample and reference beam.
Double-beam in space spectrophotometer