DNA STRUCTURE & REPLICATION
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What are the 3 major roles of DNA?
Storing, copying, and transmitting information
DNA (Deoxyribonucleic Acid)
A nucleic acid (made up of nucleotide) joined into long strands/chains by covalent bonds
What does the Nucleotide contain?
5-carbon sugar (deoxyribose), Phosphate group, Nitrogenous base
What are the four nitrogenous bases?
Adenine, Guanine, Thymine, Cytosine
How does the shape of the DNA form
There are 2 strands that are antiparallel from one another: weak hydrogen bonds form between nitrogenous base to allow separation to happen
What are the four major scientists that help research deeper into DNA?
Charagraff, Franklin, Watson & Crick
What was Charagraff’s discovery?
Found out certain nitrogenous bases always bonded to one another (Adenine & Thymine|Guanine & Cytosine)
What was Franklin’s discovery?
Used a technique called X-ray diffraction to see the double helix structure finding out that there are two strands in the DNA structure
What was Watson & Crick’s discovery?
They used Franklin’s discovery to figure out the shape of DNA is a double helix
Before duplicating DNA, what does DNA go through?
DNA goes through S-phase which replicates the DNA to pass on the info to their daughter cells
During the replication process, what happens?
DNA unzips in the middle breaking the weak hydrogen bonds creating 2 identical DNA molecules
What are the importance of enzymes?
Help unzip the DNA, adds the in the complementary nucleotide to produce a new strand of DNA, forms covalent bonds between nucleotide
Helicase
An enzymes that unwinds DNA
RNA Primase
Adds a few Nitrogenous bases to the new strand to help DNA polymolerase to continue the rest of the pattern
DNA polymolerase
Adds in the rest of the nitrogenous bases to complete the DNA (this is more easier if it’s adding on the leading strand)
Leading strand
Goes from 5’ to 3’
Lagging strand
Goes from 3’ to 5’ (antiparallel from the leading strand)
DNA ligase
Mends pieces together