MAJOR CLINICAL ENZYMES IIi
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more specific in acute pancreatitis a. amylase b. lipase c. ggt
lipase • more specific in AP • not affected by renal disorders
which of the ff methods uses a triolein as a subtrate a. wacker et al b. oliver rosalki c. cherry crandal d. saccharogenic method
Lipase methods of determination I. Cherry crandal method - substrate : OLIVE OIL modifications : used TRIOLEIN as a substrate II . turbidimetric more raid and simpler III. colorimetric method
which of the ff enzymes acts on a substrate in only in emulsified form a. amylase b. starch c. lipase d. acp
lipase • enzymes that hydrolyzes glycerol esters of long chain fatty acids • acts on a substrate in only in emulsified form • Full activity in the presence of bile salts amd colipase
newborn screening marker a. G6PD b. Cholinesterase c. GGT d. ACE
G6PD
important role in diagnosis and management of organophosphate poisoning a. G6PD b. Cholinesterase c. GGT d. ACE
Cholinesterase • important role in diagnosis and management of organophosphate poisoning ( insecticise / pesticide posioning) low CNS • used to monitor the effect of muscle relaxant after surgery
which of the following uses only Acetylcholine as a substrate a. TRUE cholinesterase b. PSEUDO cholinesterase
TRUE cholinesterase USES ONLY acetylcholine PSEUDO hydrolyzes a VARIETY of choline esters
which of the following found in serum and in the white matter of the central and peripheral nervous tissue a. TRUE cholinesterase b. PSEUDO cholinesterase
true choline • primary location are synapses of nerve cells. • also seen in RBCs, lung, brain and spleen Pseudo choli • found in serum and in the white matter of the central and peripheral nervous tissue • also in heart , liver and pancreas