(1) General Chemistry & QA
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Recognize the following category of glassware and its use: Kimax/Pyrex-borosilicate
All purpose uses; high thermal resistance.
Recognize the following category of glassware and its use: Corex-aluminosilicate
Centrifuge tubes and thermometers; resists scratching; high thermal resistance.
Recognize the following category of glassware and its use: Vycor
Acid and alkaline resistant; high thermal resistance.
Recognize the following category of glassware and its use: High silica
Cuvettes and thermometers, optically pure; high temperature changes.
Recognize the following category of glassware and its use: Low actinic
Ambered color.
Recognize the following category of glassware and its use: Flint-soda lime
Disposable.
Explain the significance of an etched ring near the top of a glass pipette.
It signifies that the pipette must be blown out after sample delivery in order to get the full volume.
Describe the types and uses of the following measuring and transfer pipette: Mohr
Graduations do not extend to the tip, not a blow out.
Describe the types and uses of the following measuring and transfer pipette: Serological
Graduations throughout the stem of the pipette, blow out-etched ring.
Describe the types and uses of the following measuring and transfer pipette: Micropipette
Volume < 1 mL.
Describe the types and uses of the following measuring and transfer pipette: Volumetric
Bulblike enlargement in the stem, single volume delivery; not a blow out.
Describe the types and uses of the following measuring and transfer pipette: Ostwald-Folin
Larger bore for viscous fluids, blow out-etched rings.
Describe the types and uses of the following measuring and transfer pipette: Measuring or graduated
Dispense several different volumes and used repeatedly for a solution; used for transferring reagents and making dilutions.
Describe the types and uses of the following measuring and transfer pipette: Transfer
Dispense one volume; greatest degree of accuracy and precision, used for diluting standards, calibrators, patient samples, or quality control material.
Describe TC pipettes.
greatest accuracy, are designed to contain an exact fixed amount of solution. They are rinsed out.
Describe TD pipettes.
delivers an exact amount. They do not rinse out.
At what temperature should pipettes be calibrated?
20 - 22C.
Describe the designation for manufactured glassware including pipettes.
Class A specifications are defined by NIST ensuring the most accurate volume of liquid delivery.
Discuss calibration of automated pipettes.
• Non-Class A pipettes must be verified by calibration. • Gravimetric procedure where 1 mL of water = 1 g at 4C. • Pipettes are checked quarterly at room temperature by weighing the volume of water delivered by the pipette.
What type of micropipette uses a piston device to facilitate aspiration and ejection of liquids with disposable tips that are changed with use?
Air Displacement Micropipette.
What type of micropipette uses a capillary tip made of glass or plastic with a Teflon-tipped plunger to transfer liquid?
Positive Displacement Micropipette.
What is the cleaning method of glassware for heavy metals or molecular assays?
Acid washed glass using dichromate and nitric acid. Final rinse with reagent grade water.
List the common types of resins used in plastic ware i the clinical laboratory and their uses.
Polystyrene, polyethylene, polypropylene, Tygon, Teflon, polycarbonate, polyvinyl chloride-tubing.