Food Analysis - Determination of Fat in Meats
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Refractometry is used in the food industry in
measuring sugar water concentrations
What refractometer do organic chemists use and why?
the Abbe refractometer to determine the RI of a material to ID or see how pure it is
What was the aim of the prac?
to determine the amount of fat in different meats
Refractive index
dimensionless number that describes how light, or any other radiation, propagates through that medium
refractive index equation
n = c/v c is the speed of light in vacuum and v is the speed of light in the substance.
RI of fat
1.47
RI bromonapthalene
1.65
Anhydrous
containing no water
How to determine % fat
= 100 Vd (n1 - n2)/w (n2 - n) V= millilitre of bromonapthalene d = density of fat n1 = refractive index of fat n2 = refractive index of the sample w = weight of sample taken