biology 5
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bacteria classification factors
• gram status • taxonomic status • oxygen use
gram status
• gram positive (purple) • gram negative (red)
taxonomic status
• genus and species • names may change • genome sequencing helps identify correctly
oxygen use
aerobic - needs oxygen anaerobic - cannot survive with oxygen facultative - can live with or without oxygen
gram negative bacteria
• stain red • thin peptidoglycan layer • have an outer membrane w endotoxins • more resistant to antibiotics
why are gram negative bacteria dangerous
• outer membrane protects them • harder to kill • resistant to penicillin
gram negative cocci (round)
• neisseria gonorrhoeae • neisseria meningitidis • moraxella catararhalis
gram negative bacilli (rods)
• haemophilus influenza • legionella pneumophila • escherichia coli
gram positive bacteria
• stain purple • thick peptiglycan wall • no outer membrane • easier of immune system to detect • more sensitive to penicillin (but resistance exists)
gram positive cell wall
• upto 90% peptidoglycan • S layer helps with protection and adhesion
gram positive cocci
• streptococcus pneumonia • staphylococcus aureus • enterococcus
gram positive bacilli (rods)
• bacillus anthracis • listeria monocytogenes
bacterial growth phases
• lag phase • log phase • stationary phase • death phase
lag phase
• bacteria adjust to environment • little to no division
log (exponential) phase
• rapid cell division • population increases fast
stationary phase
• growth slows • nutrients run out • cell division is = cell death
death phase
more cells die than are produced
what are viruses
• submicroscopic • considered non living because it needs a host to produce
what are viruses made of
• genetic material (DNA or RNA) • protein coat (capsid) • sometimes a lipid envelope • surface spikes for attachment
virus replication cycles
• lytic cycle • lysogenic cycle
lytic cycle
• virus enters cell • uses cell to make new viruses • cell bursts • cell dies
lysogenic cycle
• viral DNA inserts into host DNA • virus stays dormant • can later switch to lytic cycle
how viruses enter cells
• attachment to specific receptors (absorption) • entry into cell • release of viral genome • replication using host machinery • assembly of new viruses • release - if a cell lacks the correct receptor it cannot be infected
viruses use host
• ATP • ribosomes • amino acids • enzymes