Liver Function Test (ACP | P)
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What is the meaning of ACP?
Acid Phosphatase
What are the functions of ACP?
1) To diagnose the prostatic carcinoma 2) It is a more accurate in the dx of advanced prostatic cancer than the early dx 3) It can be used / done on vaginal swabs in rape cases
Why can ACP be used on vaginal swabs in rape cases?
Because the seminal fluid is rich in acid phosphatase
What are the tissue sources of ACP?
1) Prostate 2) Other sources: bone, liver, spleen, kidneys, RBCs, and bone marrow 3) Rare: breast carcinoma
What are the sx considerations for ACP determination?
1) ACP is unstable if the pH is 7.0 > 2) Rectal examination, prostatic massage, urinary catheterization, or instrumentation of the prostate in the previous 2 days before the test may give false high values
What is the principle of rxn for the method for ACP?
Alpha(sign) Naphthylphosphate + H2O (via the action of ACP) -> alpha(sign) Naphthol + Phosphate Alpha(sign) Naphthol + Fast Red TR Salt -> Diazo dye complex
What should be done to the ACP working rgnt?
It should be reconstituted
What are the components of the ACP working rgnt?
1) Acid phosphatase rgnt 2) L-Tartrate rgnt 3) Acetate Buffer
What are the components of ACP rgnt?
1) alpha(sign)-Naphthylphosphate 2) Na citrate 3) pH
What are the concentrations / values of the ff components of ACP rgnt (in case of reconstituted rgnt): 1) alpha(sign)-Naphthylphosphate 2) Na citrate 3) pH
1) 3 mM 2) 60 mM 3) 5.3 +/- 0.1
What are the components of L-Tartrate rgnt (in case of reconstituted rgnt)?
1) Sodium L-Tartrate 2) Citric acid 3) Na citrate 4) pH
What are the concentrations / values of the ff components of L-Tartrate rgnt (in case of reconstituted rgnt): 1) Sodium L-Tartrate 2) Citric acid 3) Na citrate 4) pH
1) 2 M 2) 70 mM 3) 10 mM 4) 5.3 +/- 0.1
What is the concentration of acetate buffer?
5 M
What is the component of acetate buffer?
pH
What is the value of pH (as a component of acetate buffer)?
5.0
What is the process (or steps) for total ACP determination?
1) Label test tubes (blank, control, and unknown) 2) Pipet 1,000 uL ACP rgnt 3) Add 10 uL L-tartrate to all tubes 4) Add 100 uL sx to all tubes 5) Incubate for 5 mins 6) After 5 mins, read and record A at 405 nm
What is the normal reference range for normal total ACP?
0 - 3 U/L
What are the causes of increased concentration of serum ACP values?
1) Metastasizing carcinoma of prostate 2) Bone disease (Paget’s disease or female breast cancer that has metastasized to bone) 3) After prostate massage
What is the cause of decreased concentration of serum ACP values?
No clinical significance to low concentration of serum ACP