MICROBIO EXAM 3
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what is a genome
all the genetic elemeents present in an organism
what is a chromosome
main geneetic element in the cell
What is a plasmid
self replicating extrachromosomal genetic element
geneotype
set of genes an organism has
phenotype
effects or characteristics an organism displays
function of sigma factor
recognize promoter region
function of rho dependenet termination
rho attaches mRNA and influencing polymerase to fall offf
what is factoir independent
this does not use rho and forms an inverted repeat of Urich region. it is weak and polymerase falls of
Operon
genes ordered one after another with one promoter
polycistrionic mRNA
open reading frame=coding region=genes
constitutive genes
always expressed
effect of sRNAs
antisense RNA PREVENT or promote translation prevent or promote degradation
what is a global regulatory system
affects many genes and pathways at one time
what is a mutation
heritage change in dna
what is reversion mutation
mutation back to wildtype genotype
suppresor mutation
mutation back to wild type genotype at a secondary site
hfr strands
high frequency of recombinants
DEscribe electrophoresis
it is an electric field across agarose gel. DNA is negatively charged and migrate towards positive electrode. small pieces migrate faster
what is the importance of southern blot
uses nucleic acid probe to detect DNA fragment