Immunochemical Techniques

. The strength of binding between an antigen and antibody is related to the a. Goodness of fit between the epitope and the F(ab) b. Concentration of antigen and antibody c. Source of antibody production, because monoclonal antibodies bind better d. Specificity of the antibody

In monoclonal antibody production, the specificity of the antibody is determined by the a. Sensitized B lymphocytes b. Myeloma cell line c. Sensitized T lymphocytes d. Selective growth medium

Which unlabeled immune precipitation method in gel is used to quantitate a serum protein? a. Radial immunodiffusion b. Double diffusion c. Counterimmunoelectrophoresis d. Immunofixation electrophoresis

In immunofixation electrophoresis, discrete bands appear at the same electrophoretic location, one reacted with antihuman IgA (α-chain– specific) reagent, and the other reacted with antihuman λ reagent. This is best described as a. An IgA λ monoclonal protein b. An IgA λ polyclonal protein c. IgA biclonal proteins d. Cross-reactivity

In nephelometry, the antigen–antibody complex formation is enhanced in the presence of a. Polyethylene glycol b. High ionic strength saline solution c. Normal saline d. Complement

Which homogeneous immunoassay relies on inhibiting the activity of the enzyme label when bound to antibody reagent to eliminate separating free labeled from bound labeled reagent? a. EMIT b. CEDIA c. MEIA d. ELISA

In flow cytometry, the side scatter is related to the a. Granularity of the cell b. DNA content of the cell c. Size of the cell d. Number of cells in G0 and G1

You analyze the DNA content on a sample of breast tissue for suspected malignancy using flow cytometry and get the following results: DI = 2.5 and % cells in S phase = 29%. Based on these results you can conclude a. These results are likely indicative of a malignant breast tumor b. This is normal breast tissue c. These results are consistent with a mostly diploid population d. The results are not consistent with one another; no information is gained

The nucleic acid technique in which RNA is converted to cDNA, which is then amplified, is known as a. RT-PCR b. PCR c. RFLP d. In situ hybridization