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E. coli calculation formulas
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卡片总数: 5 张•内容版本: v3•公开卡包•更新时间: 8/1/2026
卡片预览 (5 张)
#1
正面 (问题)
Transformation efficiency
背面 (解答)
Total number of colonies growing on the LB/amp per μg of plasmid DNA used for the transformation
#2
正面 (问题)
Initial amount or mass of plasmid DNA
背面 (解答)
Total amount of DNA (μg) = concentration (μg/μl) X volume (μl)
#3
正面 (问题)
Fraction of DNA solution spread
背面 (解答)
Volume of solution spread on plate divided by total amount in tube = fraction spread
#4
正面 (问题)
Mass of DNA spread on the experimental plate
背面 (解答)
Total mass of DNA [μg] X fraction spread = Mass of DNA spread
#5
正面 (问题)
Number of colonies per μg of plasmid DNA
背面 (解答)
Number of colonies observed divided by mass of DNA spread = Transformation efficiency