Sodium

β-galactosidase catalyzes the conversion of o-nitrophenyl-β-D- galacatopyranoside (ONPG) to o-nitrophenol and galactose. The activity of β-galactosidase depends on the sodium concentration in the sample. The absorbance increase at 405 nm is proportional to the sodium concentration in the sample.

Enzymatic photometric test

Plasma or serum

THAM buffer ph9.0 Chelator 0.15% ß-galactosidase 0.01%

Tham buffer ph8.8 ONPG

• The sodium test is very susceptible to sodium contamination. The sole use of ultrapure glass ware and disposable material is strongly recommended. • In very rare cases, samples of patients with gammopathy might give falsified results [7]. • Please refer to the safety data sheets and take the necessary precautions for the use of laboratory reagents. For diagnostic purposes, the results should always be assessed with the patient’s medical history, clinical examinations and other findings.

Ascorbic acid Conjugated and uncojugated bilirubin Lipemia Hemoglobin Calcium Copper Iron Lithium Magnesium Potassium Zinc

2.30

135-145 mmol/L

405 nm

100-180 mmol/L

42 mmol/L